IFNG ELISA Kit (GWB-SKR076)

Name Mouse IFN-gamma ELISA Kit (GWB-SKR076) Related Product Names Interferon gamma Kit Range 23-1500 pg/mL Sensitivity 23 pg/mL Sample Type Cell lysates, sera and plasma Kit Detection Colorimetric 450 nm Gene Symbol IFNG Gene Family IFN Gene Fullname interferon gamma Gene id 15978 Alias Symbols Ifg, IFN-g, IFN-gamma, Interferon gamma Swissprot ID P01580 Protein Name Interferon gamma Description of Target This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor w Kit Principle The Aviva Murine IFN-γ ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIFN-γ concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IFN-γ cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. Kit Component

Components	Amount
Capture Antibody Coated Microplate	96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP	12 mL
Ready-to-Use Substrate	12 mL
Stop Solution	12 mL
Wash Buffer (15x)	50 mL
Protein Standard Diluent	12 mL
Sample Diluent	12 mL
Detection Antibody Diluent	12 mL
Biotin-Labeled Detection Antibody	Lyophilized
Protein Standard	Lyophilized (100 ng)

Protein Accession Num NP_032363.1 Nucleotide Accession Num NM_008337.4 Assay Info Quantitative Colorimentric Sandwich ELISA Standard Range 23-1500 pg/ml Product Format 1 x 96-Well Plate or 12 x 8-Well Strips Specimen/Volumes Refer to Manual Specificity The Murine IFN-γ ELISA is capable of recognizing both recombinant and naturally produced Murine IFN-γ proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Rat: IFN-γ
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IFN-α, IFN-β, IFN-γ, IFN-λ1, IFN-λ2, IL-10, IL-18
• Murine: IFN-λ2, IL-10, IL-12, IL-18
• Rat: IL-10 Application ELISA GI Number 15978 Format ELISA Kit. Refer to manual for specific component formats Stability 6 months Reconstitution and Storage 4C/6 Months Reactivity Mouse Kit Size 12 x 8-Well Microstrips Datasheets/Manuals Printable datasheet for GWB-SKR076 Storage 4C/6 Months Species Reactivity Mouse Lead Time Domestic: within 1 week delivery | International: 1 week Intended Use Research Use Only