Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant IFN-gamma as the immunizing antigen.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, WB
  • APPLICATION NOTE: ELISA:
    In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ when used with our biotinylated antigen affinity purified anti-human IFN-γ (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL.
    Western Blot:
    To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.0-3.0 ng/lane, under non-reducing conditions.

Properties

  • PURIFICATION: This IgG1k antibody was purified on a Protein A affinity column.
  • CLONALITY: Monoclonal
  • PHYSICAL STATE: Lyophilized
  • STORAGE CONDITIONS: IFN-γ lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Additional Info

  • NCBI OFFICIAL SYMBOL: IFNG
  • ADDITIONAL NAMES: AF1, Interferon gamma receptor , Interferon gamma transducerInterferon gamma, Immune interferon, IFN-gamma, IFG, IFI
  • Protein Accession Number: P01579
  • PROTEIN GI NUMBER: 124479
  • NCBI GENE ID NUMBER: 3458
  • USER NOTE: Centrifuge vial prior to opening.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: This product is for research use only.
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