Will mapping Read 1 in Single Cell 3' sequencing increase mapping accuracy or not?
Though its low quality bases after varying-length ployT issue, will the Read 1 be helpful for mapping accurency to add this information after trimming
A related discussion about 5` sequencing is here for STAR.
"Cell Ranger has a "PE" mode that gets activated when the barcode read (read 1) is longer than 50 nt - and so it gets used for both cDNA and barcode." as indicated in the issue above.
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