I tried Wilcoxon test, t-test and negative binomial test across different amount of cells (biological replicates) (3, 6, 10, 50, 100, 500) derived from scRNA-Seq data. My intuition is that, since Negative Binomial distribution can better describe the expression distribution, it can detect more DEG than other two tests. However, it turned out Negative Binomial test always detect less DEG no matter the No. replicates is 6, 10, 50 or 100. Does this make sense? Can anyone share some opnion on this？ Thanks in advance.