why my volcano plot looks strange?

1

Hi all,
I am dealing with an LC-MS Metabolomics data. I perform log transform, quantile normalization and use linear model to regress out batch. And use wilcox test to figure out which metabolite is significantly changed.
However, the pvalue and log2 fold change of metabolites are weird. There are lots of dots near the middle line. Please help and give me some advice!
the volcano plotvolcano plot after the y-axis is reverse and loged


wilcox.test


RNAseq


Metabolomics


LCMS

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