Why my knocked-out gene is not differentially expressed in RNA seq result?!

1

Hi,
I have done RNA-seq for my Ko and Wt samples. However, the knocked-out gene is not differentially expressed. I have checked it by qPCR before and it is significantly downregulated. How can this be possible?
I also have another set of Ko, Het, and Wt mice bought from Jackson and have the same problem with them. The knocked-out gene is not differentially expressed!!!


DEseq2


RNA-seq


Knock-out

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