Why clusters look different in single nuclear RNA Seq.

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Hello,
I am analyzing single nuclear RNA mouse tissue samples (three conditions). I forced cells to 3000, ran Seurat and my DimPlot( reduction by “umap”) shown below looks very different in the condition_3. My question is, what could be the possible reasons that the condition_3 looks so different in cluster shape?

Thank you!

    condition_1         condition_2         condition_3

enter image description here


snRNA


clustering


seurat

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