Why clusters look different in single nuclear RNA Seq.
I am analyzing single nuclear RNA mouse tissue samples (three conditions). I forced cells to 3000, ran Seurat and my DimPlot( reduction by “umap”) shown below looks very different in the condition_3. My question is, what could be the possible reasons that the condition_3 looks so different in cluster shape?
condition_1 condition_2 condition_3
• 14 views