Question: What tools do you use to find potential off-target sites when designing Cas9 RNA-guided endonuclease?

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2 hours ago by

USA

Has anyone tried other tools beyond Cas-offinder to find potential off-target sites when designing sgRNAs?
My primary concern with Cas-offinder is that it overlooks indels. Are there tools that use BWA, Bowtie2, or similar software to list potential off-target sites?



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