What is the well-recognized standard of coverage uniformity
We are working on a project of developing CNV caller. We believe that efficacy of hybrid probe (targeted sequencing) is one of the factor that may affect degree of bias of depth. We therefore would like to check if the coverage uniformity of our samples is within acceptable range. My question is what is the well-recognized standard of coverage uniformity?
Also, my instinction is that, theoretically, CNV calling requires more stringent criteria of coverage univormity than SNV calling since it is more vulnerable to bias of depth. Does my impression make sense?
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