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2 hours ago by

I have been trying to use SNPeff on Galaxy to annotate a vcf file. I am working with the genome of Mycobacterium tuberculosis, and hence my reference genome is H37Rv.

Since I was unable to find a suitable speffdb, I have been trying to create my own one. I keep choosing the annotation genbank file from NCBI, in the snpeff build tool. However the database created has only 2 lines: Name and Snpeff version.

Why is this happening? Is there any other way to annotate SNPs for Mycobaterium tuberculosis using Galaxy?

I am bioinformatics newbie. Any help will be appreciated.

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