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2 hours ago by


Hello All,

I have obtained some scRNAseq data from a repository and the data are in count.matrices.rds files, which appear to be created using dropEst with "-V" option, so each file contains "exon", "intron" and "spanning" (exon/intron spanning) lists of counts.

I was wondering if anyone could advise me on how to turn these lists into a Seurat object? I am used to have "counts", so I'm not sure how to obtain such data from these 3 lists - do I merge them and then use as.Seurat function to turn the result into a Seurat object? If so, how should I merge them?

Thank you very much for your help and advice!


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