gravatar for Payal

2 hours ago by

Hi,

I have two groups with 3 replicates each for differential gene expression analysis.

Control
- Sample1
- Sample2
-Sample3

Experiment
-Sample1
-Sample2
-Sample3

However, Sample3 in Control has high duplication rate almost double reads than other samples. For differential gene expression in Deseq2, can I exclude the Sample3 in control and compare Controls (Sample1, Sample2) vs Experiment (Sample1, Sample2, Sample3). Will Deseq2 library size normalization take care of unequal sample sizes?

When I am doing DGE with n of 3 I get really low number of significant DGEs (Differentially expressed genes). But when I do n of 2 I get much higher DEGs. Or should I report differential expression results for n of 2 only. Is n of 2 statistically significant?

Thanks,
Payal

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modified 1 hour ago

by

ATpoint32k

written
2 hours ago
by

Payal40



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