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2 hours ago by

there is an error occuring after the following command

[E::hts_idx_push] Unsorted positions on sequence #4: 27130750 followed by 27130362

samtools index: failed to create index for "1_sorted.bam"

and my samtools sort command is

samtools sort 1_raw.bam -n -m 2G [email protected] 4 -o 1_sorted.bam

[bam_sort_core] merging from 8 files and 4 in-memory blocks...

I want to know where is the problem,who can give we some advice on the error??

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