SubRead featureCounts Results Total Aligments:0

0

Hello everyone, I have human Rna-Seq data reading by MiniOn.I aligned with the human reference genome (GRCh38.p13) from that website= www.gencodegenes.org/human/ using minimap2.

I download annotation from the same website. I used SubRead for quantification and feature count. On the SubRead results, there is a problem with one of the bam files. I aligned again that fastq files with the same reference genome of the first one and same gtf file and also I checked fastq file of second one but I didn't see any problem.

Do you have any suggestions for that problem?

||    Total alignments : 11214480                                             ||
||    Successfully assigned alignments : 4051945 (36.1%)                      ||
||    Running time : 2.67 minutes


||    Total alignments : 0                                                    ||
||    Successfully assigned alignments : 0                                    ||
||    Running time : 2.89 minutes


Annotation


Genome


HG38


Rna-Seq


SubRead

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