SubRead featureCounts Results Total Aligments:0
Hello everyone, I have human Rna-Seq data reading by MiniOn.I aligned with the human reference genome (GRCh38.p13) from that website= www.gencodegenes.org/human/ using minimap2.
I download annotation from the same website. I used SubRead for quantification and feature count. On the SubRead results, there is a problem with one of the bam files. I aligned again that fastq files with the same reference genome of the first one and same gtf file and also I checked fastq file of second one but I didn't see any problem.
Do you have any suggestions for that problem?
|| Total alignments : 11214480 || || Successfully assigned alignments : 4051945 (36.1%) || || Running time : 2.67 minutes || Total alignments : 0 || || Successfully assigned alignments : 0 || || Running time : 2.89 minutes
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