single tumor vs multiple normal sample differential gene expression (RNA-Seq ) analysis using DESeq2

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Hi,
I am new in bioinformatics and have a tricky problem to solve. I downloaded multiple RNA-seq datasets from TCGA GDC. My goal is to find upregulated and downregulated genes in each tumor sample after comparing it with all normals samples, e.g., "single tumor" vs. "all normal sample count" or "single tumor" vs. "single normal sample count"). This is needed to develop a model for finding new targets in cancer treatment. I looked for DESeq2 and followed a few tutorials in the last 3 days, but none worked with the single tumor sample. Can anyone guide me, how to do it?
I really appreciate any help you can provide.
Thank you
Sumit


RNA-Seq


DESeq2

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