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3 hours ago by

I came across a dataset I was going to use for some Chip-seq (chomatine) analysis, however one SRA entry for one sample has several runs:

example: www.ncbi.nlm.nih.gov/sra?term=SRX5827105

(from www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE131140)

The runs are 800M bases each which makes me believe that I should concatenate all since they belong to 1 sample. Just not clear to me why this data is split.

Doing alignment and peak calling: Can I concatenate the files before alignment, or should I concatenate only before peak calling (..... input files are split in the same way) or since they are at random: can I just pick some as a test?

Kind regards! Coulnd't really find any documentation on this! Thanks in advance!



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