RNAseq data analysis of pooled data

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Hello experts,

Kindly request you share your thoughts and tutorials for RNAseq data analysis from Raw data (FASTQ files) to arrive at differentially expressed genes.

I have brief understanding of theory based on this and this it would really helpfully if anyone could share tutorials/code for the same.

What is the best approach for pooled data? some of the posts here in the forum recommends not using pooled data but in this case i have to. so kindly share your thoughts on how to modify or alter the workflow/code/approach since i am using pooled data

Any help would be much appreciate it

Thank you for your time


DESeq2


mapping


featurecounts


clustering


RNA-Seq

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