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2 hours ago by



usually I using a pipeline that I programmed thanks to the book RNAseq by sample. For the differential expression analysis step this book provide 3 scripts, for deseq1, deseq2 and edgeR methods. All the data that I had analyzed until now have the same number of samples in the two groups. Now probably I will have data with different number of samples in each groups, so it is possible do the analysis with deseq1, deseq2 and edgeR or I need the same number of replicates in the groups?

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