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2 hours ago by

I have run MagicBLAST on a de-novo assembled metagenomic RNASeq dataset (using MEGAHIT). The contigs were queried against a specific database of genomes.

Now I am stuck as to how to visualise the hit results from MagicBLAST (.sam file). I could write a python notebook to decode the MagicBLAST results (get nucleuotide start, stop and sequence for query against reference) and then plot as a colour-coded horizontal ~'bar' type plot showing reference vs contig overlap. But I expect there is already open source software that can do this.

IGV is a good example of what I am trying to do- but as far as I can tell you can only show one reference sequence. I want to plot multiple reference sequences (eg 10 - to save time) and for each show the contigs which overlap with each refseq. Is there an open source tool or script which already does this?

What are examples of workflows (on desktop linux, prefer local to web-based tools) once you have generated blast against ref sequences? (I am a novice)- aim is to identify genomic coverage of specific genes in metagenomic datasets.

A related question is what is the advantage of de-novo assembly vs alignment against specific sequences? I am struggling to work out what to do with the de-novo data


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2 hours ago


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