RNA_Seq data aligned used uniquely or multi mapped reads impact on result interpretation

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I have some transcriptomic (Whole) sequencing data that I should analyse. I would like to do raw data alignment to a reference genome taking into account the multi mapped reads and uniquely mapped reads. So my questions are :

1- Considering unique and multi-aligned reads, wouldn't this be a source of bias in my results interpretation?

2- By hiding the multi-mapped reads, could I lose information on the expression of important genes?

thank you in advance


NGS


Transcriptomic


GENOMIC

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