RNA sequence analyzing data using R after DESeq2 (illumina)
I aligned 3 group data and used DESeq2 in basespace.illumina.com/analyses/
Now I excel file of data after DESeq2.
can you guide how to continue in R and draw a plot Heatmap, and other graphs for the study of gene expression of 3 groups?
1- how upload excel with the below information in R?
2- when I use dds, there is an error. how I can remove the error? Thanks in advance
dds <- DESeq(dds) Error in is(object, "DESeqDataSet") : object 'dds' not found baseMean log2FoldChange lfcSE pvalue padj ## <numeric> <numeric> <numeric> <numeric> <numeric> ## FBgn0000008 95.14429 0.00119920 0.151897 0.9918817 0.997211 ## FBgn0000014 1.05652 -0.00473412 0.205468 0.8172987 NA
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