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3 hours ago by

Hello All,

I want to do RNA seq analysis for multiple files together without any replication and control. I do not want to perform a comparison with control, I want to basically run a linear regression. I have input files (RNA paired end) for 50 varieties. There are 15 chemical compounds concentration each for all 50 varieties. I am using salmon to create TPM values and then I want to test for regression relationship between independent variable (each chemical compound which is a vector of all 50 varieties) and dependent variable as TPM values from all the varieties. I have generated the TPM values in quant.sf files using salmon and I need some help about the steps to move forward to perform linear regression analysis in R as this is my first time doing it.

Thank you in advance for any suggestion/comment/guidance!

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