rMATS BAM file MemoryError Issue


Hi everyone!
This is my first time posting here. I have a very large BAM file (4.8GB) which I would like to process using the rMATS program to find splice variants. However, when I pass in the file through rMATS, I am receiving the following error.
MemoryError from BAM File

Some searching online revealed that the BAM file might be too big to process through the rMATS pipeline. I tried to split the BAM file by chromosome, but I end up getting many different files (I've also included a partial list of the chromosomes below).

Partial List of Chromosomes

Could someone please recommend a way for me to split the BAM file in a different manner? Thanks in advance!





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