• FastQC report analysis

    my FastQC report shows sequence length as 20-36.Can I accept the sequence?

    Basic Statistics...

  • how to remove warning and failures observed inQuality report by Fastqc for RNA-Seq data

    Hi All,

    I ran FastQC software on RNA-Seq data of Plant sample generated from illumina HiSeq pla...

  • Interpretation of FASTQC results - do I need to trim my sequence?

    I am quality checking a gene sequence using FASTQC.

    It has given me warnings about the sequence ...

  • RNASeq analysis, FastQC how to solve warning and fail

    I do a FastQC for quality control my data but i some situation there are a caution, can anyone te...

  • How to deal with the error for "Per Sequence GC Content" in FastQC tool?

    I am working on the genomic data of E. Coli. My sequence reads are of high quality, and I already...

  • FastQC low %A in per base sequence content

    Hi

    I have some Illumina RNA seq data, 75bp paired end reads. I've run FastQC & trimmomatic a...

  • Warning while finding bcftools consensus

    I am trying to find bcftools consensus using GRCH38. But I am getting the following error. What d...

  • FastQC Results -- Per Sequence GC Content and Kmer Contents

    I have 32 fastq data from whole exome sequencing. I ran FastQC on them, and the following 3 items...

  • FastQ quality check : what can we correct ?

    Hello there,

    I am having a ot of MiSeq data I am trying to analyze and I figured out using FastQ...

  • fastqc and coverage

    I have 50% duplicates on WGS on tumour samples and while I was expecting that the coverage will b...

  • Small RNA library contaminated by primers?

    Hello,

    I have to analyze differential expression on 2 SRA files containing data from a small RN...

  • trimming fastq files with Trimmomatic

    Dear all,

    "TO TRIM OR NOT TO TRIM?"

    My PE RNAseq library prep of human brain tissue was made wi...

  • How to repair *all* problems identified by FastQC?

    hello,

    I am downloading public data, and am running FastQC on a number of FASTQ files I've downl...

  • Tophat : Read Length Warning

    Hello,
    I am running tophat over reads with length equal to 36 to 38. And while running it the bel...

  • Quality report by Fastqc, Result Interpretation and Next step parameters

    Hi All,

    I ran FastQC software on whole genome sequence (WGS) data of Human sample (with expected...

  • meaning of the Per tile sequence quality

    Hello every body

    I wonder if any body tell me about  Per tile sequence quality?

     

  • Interpreting Fastqc Results For Chip-Seq Data

    Hello,

    I am processing a ChIP-seq experiment I downloaded from GEO (link). The SRA files are mas...

  • Mauve export ortholog difficulties

    Hello

    After using Illumina and de novo assembly on 23 bacterial genomes, I have used Mauve to re...

  • Warning in Genemark and maker

    Thanks everyone
    After my training with Genemark, there are two kinds of warning in the "log" out...

  • kmer content changes after trimming and removing adapters from reads

    Dear community

    I have a large set of FASTQ files from genomic DNA. I ran them through FastQC and...

  • What is the importance of checking per base sequence content and per sequence GC content during Quality Control of NGS reads?

    Hello! I'm quite new to NGS data analysis and have a few questions about certain modules in FASTQ...



  • Source link