gravatar for O.rka

2 hours ago by

I have a very large fastq file that I want to do variant calling on. Would it make sense to remove duplicate reads before mapping using bowtie2? After this I was going to use freebayes.

What effect will removing duplicate reads have on my resulting variant file compared to keeping all of the reads?

Will I have lower confidence variant calls?

link

written
2 hours ago
by

O.rka140



Source link