R command worked yesterday, but stalls today?


I am having trouble with this MOFA Tutorial in R: raw.githack.com/bioFAM/MOFA2_tutorials/master/R_tutorials/10x_scRNA_scATAC.html

Yesterday, the commands were working but today R stalled on this particular command:

seurat <- seurat %>%
  .[,[email protected]$pass_accQC==TRUE & [email protected]$pass_rnaQC==TRUE]

I tried to redo the run but now it keeps stalling on the following command, which is to download the data:

seurat <- readRDS(url("ftp://ftp.ebi.ac.uk/pub/databases/mofa/10x_rna_atac_vignette/seurat.rds"))

R studio displays the red octagonal symbol at the top right corner, indicating it is running the code, but never completes the command. Both commands were working fine yesterday but I haven't been able to get it to work today or figure out what the issue is yet. Do you have any suggestions?



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