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2 hours ago by

I have 100 RNAseq files from 100 samples. using bioinformatics methods, I want to do final quality control and check if any technical problem in the data or not. since the files do not have annotation (treatment is not clear to me), I think PCA is not useful here since I do not know whether the samples that are clustered together have similar treatment or not (annotation is not clear). so the question is that what are the bioinformatics way to check if any technical problem happened (specially during sequencing)?

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ATpoint31k

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2 hours ago
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Sara90



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