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14 hours ago by

United States

For those replicates that are very different from each other, you may find it helpful to plot simple X-Y scatter plots. This will tell you if the variance is happening for all ranges of read counts, or only for low-read count genes, or only high-read count genes, etc...

If the replicate scatter plots are "all over the place", I would then dig into if you think this is experimental error, or if this is actual variation due to biology.

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14 hours ago
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jerry70



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