public tools to distinguish germline variants from somatic ones in NGS data without matched normal

Hi all,

Distinguishing germline variants, especially those pathogenic ones, are of great importance, since these variants might increase the cancer susceptibility in other family members.

It is quite challenge when blood sample/matched normal is absence, leaving a lot of variants with zygosity/Allele frequency around 0.5.

Is there any public tool to determine the origin of the variants?

Thanks

Junfeng