Would anyone in the community have a pre-mRNA reference GTF they would be willing to share? (mm10 and GRCh38)
Or could point me towards the direction of finding one if they are aware if someone has made theirs available online?
I'd like to be able to map my data to be able to detect introns/exons (spliced/unspliced) transcripts to use with RNA velocity.
Ideally the CellRanger pipeline.
These were already aligned by our genomics facility with the standard GTF provided by 10x that does not capture that info, so would ideally need to re-count using a GTF that holds that information. Platform = 10x genomics chromium 3' v3.