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2 hours ago by

Hi, I am trying to assemble some genomes from pacbio reads using HGAP3 followed by quiver polishing.
However, even after multiple rounds of quiver polishing, I cannot attain quality value 50 (QV50) for a genome.

QV50 seems to be recommended and I am able to get upto QV30 only. I have done polishing for almost 15 times already. I have tried polishing with different parameter changes like subread length cutoff and blasr maximum divergence.

Can anyone suggest me on how I can achieve an assembled genome of QV50 value?

Thank you

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