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2 hours ago by

I have downloaded transcriptomes from NCBI. these were Pacbio (RSII) read. But i dont know what should be best strategy to get transcripts. should i go for assembly? Or for alignment? If i go for alignment then how about the denovo transcripts? I downloaded Stringtie2. But what to input in it didnt understand clearly. Can someone please give me some clue? Thanks

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