I have few RNASeq samples where samples are carrying over expressed gene for e.g.
Sample1 = over expressed gene ABC,
Sample2 = over expressed gene DBG ... etc.
and they also have a common control(which not carrying any over expressed gene)
Now do I just perform typical RNA-Seq analysis using Hisat ---->Stringtie-----> ballgown or I need to treat these samples special and do some standardization before ?