On whether or not to normalize scRNAseq expression like qPCR
In measuring gene expression of qPCR, we usually does it this way
Where GeneX is some gene of which we need to decide.
I know there scRNAseq are usually normalize using TPM,FPKM, Deseq method etc.
But my question is whether in single-cell RNAseq we need to divide again a gene expression against another gene expression (as base).
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