On whether or not to normalize scRNAseq expression like qPCR

1

In measuring gene expression of qPCR, we usually does it this way

enter image description here

Where GeneX is some gene of which we need to decide.

I know there scRNAseq are usually normalize using TPM,FPKM, Deseq method etc.
But my question is whether in single-cell RNAseq we need to divide again a gene expression against another gene expression (as base).


single-cell


qpcr


rnaseq

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