Question: On read sequencing libraries, the opposite strands are considered on the files? (and how are they handled on assembly?)

gravatar for v.berriosfarias

8 hours ago by

Just want to know if on my paired end reads fastq files are considered the opposite strand, Does on the sequencing procedure the opposite DNA strands discarded or are they sequenced too? if the are sequenced, how are the opposite DNA strands handled on contig assembly steps?



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