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2 hours ago by

I tried to map a list of miRNAs to all mature miRNAs from mirbase.
My query file is like;

>Bany_Scaf11_1015
UAGGAACUUCAUACCGUGCUCU
>Bany_Scaf5_35602
UCUUUGGUUAUCUAGCUGUAUGA
>Bany_Scaf19_11996
UGGAAGACUAGUGAUUUUGUUGUU

I downloaded all mature miRNAs and created the database using the command;

makeblastdb -in mature.fa -dbtype nucl -out mature -parse_seqids

Then I blast;

blastn -query sorted_mature.fa -db mature -num_threads 36 -outfmt 6 -out sorted_mature_out

In the end I got an empty output file with 0 hit...anything wrong with my analysis?



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