Hi all,
I have several questions regarding to NGS miRNA analysis:
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For the NGS small RNA size distribution, the peak shall be at around 22 nt, but what if there is another small peak at 29 nt, is it normal?
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While doing the analysis on the clear read, I selected these reads that longer than 18 nt for analysis. Is that ok if I select reads at the range of 18-26 nt for the downstream analysis? Why? How this would affect the analysis results (e.g. identification of dysregulated miRNAs, control VS. treament)?
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How to decide if there is any contamination when looking at my NGS small RNA data?
Highly appreciated for your kind help!
Best,
William