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2 hours ago by

Hello!

I use ONT data

I mapped reads with minimap2: -a -x splice -k 14 and etc - next use samtools to sort

But when I go to the next step to identify splice events I have the error:

SAM validation error: ERROR: Record 1, Read name 741eead6-18e3-4fb4-baa7-b2cb28ecb1aa, CIGAR covers 2466 bases but the sequence is 0 read bases

Can you explain to me why I have this error? Maybe I have a problem with my fastq file?

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modified 2 hours ago

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Ram32k

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2 hours ago
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mailard 0



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