2 hours ago by
Hello!
I use ONT data
I mapped reads with minimap2: -a -x splice -k 14 and etc - next use samtools to sort
But when I go to the next step to identify splice events I have the error:
SAM validation error: ERROR: Record 1, Read name 741eead6-18e3-4fb4-baa7-b2cb28ecb1aa, CIGAR covers 2466 bases but the sequence is 0 read bases
Can you explain to me why I have this error? Maybe I have a problem with my fastq file?
