I was wondering if anyone know of a way to modify an annotation file, such as a gtf or gff after the reference genome was inserted with a transgene or other external snippets.
We have a Whole Genome Sequencing project, which was done on yeast genome modified with an insertion at chromosome II. I would like to modify my annotation file ( I have in this case only a gff). We know exactly where the sequence was inserted, so I need to modify the positions of all chromosomal elements from a specific position to the end of this chromosome.
I would appreciate the help, if someone knows of a simple way to do so.