gravatar for leonardo.caserta

2 hours ago by

Hello

I'm trying to align a fastq file obtained from a concatenation of reads basecalled with guppy high accuracy in MinIT. I'm receiveing the following error message:

[WARNING] wrong FASTA/FASTQ record. Continue anyway.[M::worker_pipeline::2.165*0.99] mapped 67504 sequences

[E::sam_parse1] query name too long

[W::sam_read1] Parse error at line 67541

[main_samview] truncated file.

Alignment pipeline failed.

Here's the command i used:

mini_align -i Deerpox_2.fq.gz -r /home/lcc88/Desktop/References/Deerpox.fasta -p Deerpox_2

Apparently modifying the query names longer than 252 is the solution but i have no idea how to do it. Does anyone know how to do it? Or possibly another solution for this problem.

Thank you,

Leonardo



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