Meaning of the column of sam file

1

Hello!
Referring to this columns in sam file:

  1. QNAME
  2. FLAG
  3. RNAME
  4. POS
  5. MAPQ
  6. CIGAR
  7. RNEXT
  8. PNEXT
  9. TLEN
  10. SEQ
  11. QUAL

I wanted to ask how to extract the information about end position of the read?
Morover if I want to know which strand it is, it will be depend on FLAG? If the FLAG is 16 that mean it is reverse and in the other cases it will be forward?


reads


position


sam

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