Many TLENs in a SAM file seem much too big

0

Hello.

I have a SAM file from an alignment between a human sample against an NCBI reference genome. I took the TLENs (the 9th field in each SAM file entry) from the SAM file. Some TLENs are over 240 million base pairs. Does anyone know how can reads get that long? maybe a polymerase in the some of the sequencing elongations managed to elongate a DNA just by chance.

Thanks.


NGS


SAM


comperative-genomics


alignment

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