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3 hours ago by

Wondering if anyone has run into a similar issue. I tried running lofreq with the call option:

lofreq call -s -S /path/to/dbsnp_147_b37_common_all_20160601.vcf -f 
  /path/to/Homo_sapiens_assembly19.fasta -o /path/to/output.vcf /path/to/tumor_bam.bam

However, I do not see any calls, and I only see a VCF header in output.vcf. All that is in the log is:

Number of substitution tests performed: 0
Number of indel tests performed: 0

Is there a reason why this might be?

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