gravatar for claire.lamaison

2 hours ago by

Hi, i have to analyse the differential expression of 700 genes, for patients at diag and relapse
i have normalized counts with log2
i would like the p value for each gene to perform a volcano plot and I wanted to use lmfit function from the limma package
but is it possible without any probes? i have this message:

> E=read.table("nana.csv", header=TRUE, row.names=1,sep="t", dec=".")
> A=as.matrix(E)
> F<-t(A)
> fit <- lmFit(F,design=NULL)
Error in getEAWP(object) : 
  **Data object doesn't contain numeric expression values**



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