gravatar for ccpowell

5 hours ago by

Hi there,

I am trying to run, followed by, wigToBigWig (UCSC Genome Browser script), to determine GC content and TATA boxes: is located at:

WigToBigWig at:

When I run the following command I get the following error:

$ perl 5 tomato.fa tomatoGC5.wig

Can't locate Bio/ in @INC (you may need to install the Bio::SeqIO module) (@INC contains: /home/user/perl5/lib/perl5/5.26.1/x86_64-linux-gnu-thread-multi /home/user/perl5/lib/perl5/5.26.1 /home/user/perl5/lib/perl5/x86_64-linux-gnu-thread-multi /home/user/perl5/lib/perl5 /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.26.1 /usr/local/share/perl/5.26.1 /usr/lib/x86_64-linux-gnu/perl5/5.26 /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.26 /usr/share/perl/5.26 /home/user/perl5/lib/perl5/5.26.0 /home/user/perl5/lib/perl5/5.26.0/x86_64-linux-gnu-thread-multi /usr/local/lib/site_perl /usr/lib/x86_64-linux-gnu/perl-base) at line 5.
BEGIN failed--compilation aborted at line 5.

Here is my PATH:

So it appears perl5 is in my path, perl works I run a basic perl script & I have also run:

cpanm Bio::Perl

However, I still get this error...

Is there a specific way to install or set my path to find Bio/ I don't believe I've installed it correctly. I think this is probably due to the improper installation of BioPerl but I cannot find any
good documentation.

Anything helps! Thank you!!

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