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2 hours ago by

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Hey guys
I used igv to check a WES bam file.
When I looked to EGFR gene, I saw that the refseq genes with blue thicker bar had a better coverage of reads. However there is little read on the blue thinner bar region.

I thought all blue bar region was exon. Now I don't know whether I am right.

So, the question is:
1, Does the whole blue bar mean exon region? What the exact mean of thicker bar and thinner bar?
2,Why the blue thicker bar has a better coverage ?Please help me find some possible reasons.

Thanks everyone!

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2 hours ago
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Nich10



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