gravatar for tengwen2018

3 hours ago by

Hello, I am a new hand and I encountered this problem when I was scaffolding using Juicer with HiC data. The genome size is approximately 680M with ten chromosome. Using the HiC data, I scaffold only 400M to the ten chromosome. Although, many contigs can be scaffolded to the chromosome by eyes, Juicer failed to locate these contigs to the right position. ibb.co/3zCHT8R

Next, I scaffolded these contigs manually. But I got the weird structure!
ibb.co/R2QS0bx ibb.co/G2Pn0ZZ

I have removed the duplication after canu assembling and the the remaining dups is 2%.

Please help me and thank you so much!

link

modified 3 hours ago

written
3 hours ago
by

tengwen20180



Source link