There is a question about `Seurat` that has been bothering me all the time. I would like to ask your opinions. For the case of multiple samples, In order to eliminate the batch effect, `Seurat` clustering will choose to integrate multiple samples, which is no problem. But when searching for markers, it chooses to use the raw data matrix in order to avoid the elimination of differences by data integration.However, when using Heatmap to display markers, integrated data matrix is used to plot, which leads to many differentially found genes that are not different when visualized.The same thing happens when looking for differential genes and subsequent functional analyses, such as GSVA, receptor ligand pairs, etc., should these analyses use raw or integrated data?If the integrated data is used, will it affect the identification of differences, and if the original matrix is used, will there be a batch effect?

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