Hello everyone,
I have question please
I have bam file containing unmapped reads that I want to assemble.
I tried samtools fastq to obtain the two files forward and reverse
I did this command
samtools fastq -1 wPru_1_Unmapped_reads.fastq -2 wPru_2_Unmapped_reads.fastq wPru_Unmapped_reads.bam
but when I wanted to perform the assembly (shovill) it didn't work it shows that the number of forward is larger than the number of reverse reads
The number of left read-pairs is larger than the number of right read-pairs
my objective now is how to extract forward and reverse reads from bam file
Thank you very much