How to set the number of mismatch in blastn

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Hi everyone, I'm running blast nucleotide with word-size of 6 using the following command:

blastn -query 6452.fasta -db ref -out BlastResults.txt -num_threads 8 -outfmt '6 qseqid sseqid sseq' -word_size 6

Now I want to set the number of mismatch to 2 for the command. how should I add it? I added "-penalty 2" to command but it doesn't work!

Regards.


blastn


mismatch


penalty

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